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ALB regulates immune-related gene expression at the transcriptional level in melanoma cells. (A) qRT-PCR analysis of ALB mRNA levels in <t>A375</t> and SK-MEL-28 cells transfected with control (oe-NC/si-NC), ALB overexpression plasmid (oe-ALB), or ALB-targeting siRNA (si-ALB). (B) Relative mRNA expression of IL-6, TNF-α, TGF-β, IL-17A, and RORγt in A375 cells after ALB modulation. (C) Corresponding mRNA expression profiles in SK-MEL-28 cells. Data were presented as mean ± SD (n = 3). ** P < 0.01 vs. oe-NC group; ## P < 0.01 vs. si-NC group.
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ALB regulates immune-related gene expression at the transcriptional level in melanoma cells. (A) qRT-PCR analysis of ALB mRNA levels in <t>A375</t> and SK-MEL-28 cells transfected with control (oe-NC/si-NC), ALB overexpression plasmid (oe-ALB), or ALB-targeting siRNA (si-ALB). (B) Relative mRNA expression of IL-6, TNF-α, TGF-β, IL-17A, and RORγt in A375 cells after ALB modulation. (C) Corresponding mRNA expression profiles in SK-MEL-28 cells. Data were presented as mean ± SD (n = 3). ** P < 0.01 vs. oe-NC group; ## P < 0.01 vs. si-NC group.
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ALB regulates immune-related gene expression at the transcriptional level in melanoma cells. (A) qRT-PCR analysis of ALB mRNA levels in A375 and SK-MEL-28 cells transfected with control (oe-NC/si-NC), ALB overexpression plasmid (oe-ALB), or ALB-targeting siRNA (si-ALB). (B) Relative mRNA expression of IL-6, TNF-α, TGF-β, IL-17A, and RORγt in A375 cells after ALB modulation. (C) Corresponding mRNA expression profiles in SK-MEL-28 cells. Data were presented as mean ± SD (n = 3). ** P < 0.01 vs. oe-NC group; ## P < 0.01 vs. si-NC group.

Journal: Future Science OA

Article Title: Integrative analysis of adiponectin-related genes reveals immune subtypes and prognostic significance in melanoma

doi: 10.1080/20565623.2026.2664611

Figure Lengend Snippet: ALB regulates immune-related gene expression at the transcriptional level in melanoma cells. (A) qRT-PCR analysis of ALB mRNA levels in A375 and SK-MEL-28 cells transfected with control (oe-NC/si-NC), ALB overexpression plasmid (oe-ALB), or ALB-targeting siRNA (si-ALB). (B) Relative mRNA expression of IL-6, TNF-α, TGF-β, IL-17A, and RORγt in A375 cells after ALB modulation. (C) Corresponding mRNA expression profiles in SK-MEL-28 cells. Data were presented as mean ± SD (n = 3). ** P < 0.01 vs. oe-NC group; ## P < 0.01 vs. si-NC group.

Article Snippet: Human melanoma cell lines A375 (#CRL-1619) and SK-MEL-28 (#HTB-72) were obtained from ATCC and cultured in Dulbecco’s Modified Eagle Medium (DMEM; Gibco, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, USA) and 1% penicillin–streptomycin at 37 °C in a humidified incubator with 5% CO 2 .

Techniques: Gene Expression, Quantitative RT-PCR, Transfection, Control, Over Expression, Plasmid Preparation, Expressing